Skip to Main content Skip to Navigation
Journal articles

Effects of mitogen-activated protein kinases on nuclear protein import

Abstract : ERK-2 MAP kinase activation induces inhibitory effects on nuclear protein import in vascular smooth muscle cells. The mechanism and characteristics of this effect of ERK-2 were investigated. An unusual dose-dependent effect of ERK-2 on nuclear protein import was identified. At higher concentrations (1 microg/mL) of ERK-2, nuclear protein import was stimulated, whereas lower concentrations (0.04 microg/mL) inhibited import. Intermediate concentrations exerted intermediate effects. The stimulatory and inhibitory effects at the 2 different ERK-2 concentrations were observed in both conventional, permeabilized cell assays of nuclear protein import and with in situ microinjection of smooth muscle cells. The biphasic effects of ERK-2 on import were also found for the other 2 members of the MAPK family, p38 and JNK. RanGAP was identified by structural analysis as a candidate target protein responsible for mediating the effects of ERK-2. After pretreatment with high concentrations of ERK-2, RanGAP activity was significantly increased by approximately 50%. In contrast, low concentrations of ERK-2 significantly attenuated RanGAP activity. These results demonstrate that all 3 members of the MAPK family can alter nuclear protein import in opposite directions depending upon the concentration of ERK-2 used. RanGAP represents the MAP kinase target whereby nuclear transport can be stimulated or inhibited.
Document type :
Journal articles
Complete list of metadata

https://hal.inrae.fr/hal-02657669
Contributor : Migration Prodinra <>
Submitted on : Saturday, May 30, 2020 - 10:54:13 AM
Last modification on : Thursday, July 2, 2020 - 3:12:30 PM

Identifiers

Collections

Citation

Randolph S. Faustino, Delphine C. Rousseau, Melanie N. Landry, Annette L. Kostenuk, Grant N. Pierce. Effects of mitogen-activated protein kinases on nuclear protein import. Canadian Journal of Physiology and Pharmacology, NRC Research Press, 2006, 84 (3-4), pp.469-475. ⟨10.1139/y05-131⟩. ⟨hal-02657669⟩

Share

Metrics

Record views

10