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Article Dans Une Revue Vaccine Année : 2008

Production of biologically active CXC chemokines by Lactococcus lactis: Evaluation of its potential as a novel mucosal vaccine adjuvant

Résumé

Chemokines have been described as essential mediators in leukocytes migration to inflammatory sites and to secondary lymphoid organs. Mig and IP-10 are two CXC chemokines that recruit mononuclear cells in vivo and inhibit angiogenesis. In addition to their chemotactic roles, Mig and IP-10 have also an important role in the adaptative immune response. In this study, we asked whether a food-grade bacterium, Lactococcus lactis, is able to produce a fusion protein comprising Mig and IP-10 (Mig::IP-10). The activity of the recombinant Mig::IP-10 produced by the genetically engineered L. lactis (LL-Mig::IP-10) was confirmed in a murine spleen cells chemotaxis assay. Moreover, the adjuvant properties of LL-Mig::IP- 10 strainwere evaluated in mice by the co-expression of a model antigen, the human papillomavirus type 16 E7 protein. Our data show that LL-Mig::IP-10 can produce a genetic fusion of Mig::IP-10 biologically active. This recombinant strain represents a potential candidate for the development of newstrategies for mucosal vaccination.

Dates et versions

hal-02658477 , version 1 (30-05-2020)

Identifiants

Citer

Naima G. Cortes-Perez, Luis F. da Costa Medina, Francois F. Lefèvre, Philippe P. Langella, Luis L. Bermudez Humaran. Production of biologically active CXC chemokines by Lactococcus lactis: Evaluation of its potential as a novel mucosal vaccine adjuvant. Vaccine, 2008, 26 (46), pp.5778-5783. ⟨10.1016/j.vaccine.2008.08.044⟩. ⟨hal-02658477⟩
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