Summary Author : Viruses are fast evolving organisms for which changes in fitness and virulence are driven by interactions between genomes such as recombination, functional complementation, and competition. Viruses being intra-cellular parasites, one basic parameter determines the potential for such interactions: the cellular multiplicity of infection (cellular MOI), defined as the number of genome units actually penetrating and co-replicating within individual cells of the host. Despite its importance for virus evolution, this trait has scarcely been investigated. For example, there are only three point estimates for eukaryote-infecting viruses while the possibility that the cellular MOI may vary during the infection or across organs of a given host individual has never been conclusively addressed. By monitoring the cellular MOI in plants infected by the Cauliflower mosaic virus we found remarkably ample variations during the development of the infection process in successive leaf levels. Our results reveal that the opportunities for recombination, complementation and competition among viral genomes can greatly change at different infection phases and at different locations within a multi-cellular host.