Gene expression profiling of the nitrogen starvation stress response in the mycorrhizal ascomycete Tuber borchii - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement Access content directly
Journal Articles Fungal Genetics and Biology Year : 2006

Gene expression profiling of the nitrogen starvation stress response in the mycorrhizal ascomycete Tuber borchii

Abstract

The focus of this work is on the nitrogen starvation stress responses operating in a plant symbiotic fungus. A cDNA array profiling analysis was conducted on N-limited mycelia of the mycorrhizal ascomycete Tuber borchii. Fifty-one unique transcripts, out of 2062 redundant arrayed cDNAs, were differentially expressed by at least 1.5-fold in response to N deprivation. Only two N assimilation components—a nitrate transporter and a high-affinity ammonium transporter—were found among differentially expressed genes. All the other N status responsive genes code for as yet unidentified hypothetical proteins or components not directly involved in N assimilation or metabolism, especially carbohydrate binding proteins and oligosaccharide as well as lipid modifying enzymes. A subset of cDNA array data were confirmed and extended by Northern blot analysis, which showed that most of the latter components respond not only to nitrogen, but also to carbon source depletion.

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Genetics

Dates and versions

hal-02659650 , version 1 (30-05-2020)

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Barbara Montanini, Silvia Gabella, Simona Abbà, Martina Peter, Annegret Kohler, et al.. Gene expression profiling of the nitrogen starvation stress response in the mycorrhizal ascomycete Tuber borchii. Fungal Genetics and Biology, 2006, 43 (9), pp.630-641. ⟨10.1016/j.fgb.2006.04.001⟩. ⟨hal-02659650⟩
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