Accumulation of fluorescent pigments in cooked bovine meat (M. Longissimus thoracis) was studied in relationship with the heating parameters (time and temperature). Muscles were aged at 4 degrees C for 11days under vacuum before cooking. Meat cooking was performed by applying jets of steam. Three different heating treatments were tested: two with constant surface temperatures of 65 and 96 degrees C for 300s, and one with a continuously increasing surface temperature up to 207 degrees C. After extraction in water/dichloromethane/ethanol, fluorescence pigments were distributed between the apolar phase (emission 420-440nm after excitation at 360nm) and the polar phase, where two emission peaks were seen (emission 410-430 and 515nm after excitation at 360nm). Fluorescence in the two phases was little affected by heating at the two constant temperatures while it increased exponentially after 1min of treatment, as the varying temperature reached 141 degrees C. The maximum fluorescence increases, measured in the extreme conditions of cooking (207 degrees C/300s), were of 5000% in the apolar phase and 1700% in the polar phase. Thiobarbituric acid reactive substances (TBARS) and protein carbonyls were measured in parallel. The correlations between these two parameters and the fluorescence emission demonstrated that the interaction between proteins and aldehyde products of lipid peroxidation was mainly involved in the production of fluorescent pigments in cooked meat.