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Investigation of the chromatographic process via pulsed-gradient spin−echo nuclear magnetic resonance. Role of the solvent composition in partitioning chromatography

Abstract : The diffusional properties of molecules in solution vary dramatically upon addition of a solid chromatographic phase. This effect can be monitored via pulsed-gradient spin - echo NMR used in conjunction with moderately fast rotation of the sample (high-resolution magic angle spin- ning) to produce exploitable spectra. The molecular diffusion coefficients observed in this condition are aver- ages reflecting the equilibrium population distribution among the different phases. It is thus possible to use this information for investigating a crucial step of reversed- phase chromatography, namely, the partitioning of the analyte between different phases. In this work, we de- scribe the evolution of the apparent diffusion coefficient of typical solutes for water/acetonitrile solvent mixtures of varying proportions.
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Guilhem Pages, Corinne Delaurent, Stefano Caldarelli. Investigation of the chromatographic process via pulsed-gradient spin−echo nuclear magnetic resonance. Role of the solvent composition in partitioning chromatography. Analytical Chemistry, American Chemical Society, 2006, 78 (2), pp.561-566. ⟨10.1021/ac051454n⟩. ⟨hal-02665659⟩

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