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Article Dans Une Revue Vitis Année : 2006

New rapid PCR protocols to distinguish genetic groups in Erysiphe necator

Résumé

In order to differentiate the two genetic groups (A and B) detected in populations of the powdery mildew fungus, *Uncinula necator*, we developed PCR primers to amplify two sequence characterized amplified regions (SCAR) and one sequence with a microsatellite motif (SSR). Primer pairs designed from SCARs gave a fragment for group A isolates and no fragment for group B isolates. The primer pair mO3E11 designed for the SSR sequence amplified two different sequences. The first sequence had the SSR motif and presented two alleles: one allele was present in all 49 isolates of group A tested and in two out of 34 isolates of group B, and a second allele was present in the remaining 32 isolates of group B. The other sequence did not have the SSR motif and was amplified only in the 34 group B isolates. The mO3E11 pair appears suitable for large-scale studies since it identified groups in only one PCR reaction and gave strong signals with DNA templates that can be rapidly obtained from a minute quantity of fungal material.
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Dates et versions

hal-02665770 , version 1 (31-05-2020)

Identifiants

  • HAL Id : hal-02665770 , version 1
  • PRODINRA : 16285
  • WOS : 000235008000008

Citer

Jean-Pierre Peros, Claire Troulet, Mickaël Guerriero, Corinne Michel-Romiti, Jean-Loup Notteghem. New rapid PCR protocols to distinguish genetic groups in Erysiphe necator. Vitis, 2006, 45 (1), pp.47-48. ⟨hal-02665770⟩
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