Protein polymorphism between two Picea abies ecotypes as revealed by two-dimensional gel electrophoresis and tandem mass spectrometry - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement Accéder directement au contenu
Article Dans Une Revue Journal of Heredity Année : 2008

Protein polymorphism between two Picea abies ecotypes as revealed by two-dimensional gel electrophoresis and tandem mass spectrometry

Résumé

In species with high gene flow and consequent low interpopulation differentiation over wide geographic ranges, differential gene expression along ecological gradients often reveals adaptive significance. We investigated potential differences in protein expression between Picea abies ecotypes adapted to contrasting altitude conditions. Protein expression patterns were compared between needles and roots of 2-month-old P. abies seedlings by means of 2-dimensional electrophoresis. Proteins exhibiting differential expression between the 2 ecotypes were analyzed by tandem mass spectrometry. A total of 19 proteins exhibited qualitative or quantitative polymorphism between the 2 populations. These proteins exhibited organ-specific expression, and the level of interpopulation protein polymorphism was organ dependent. Among differentially expressed proteins, we identified proteins involved in photosynthesis, photorespiration, root tracheary element differentiation, and transmitochondrial membrane transport. Our results show that P. abies seedlings from locally adapted ecotypes exhibit consistent differences in protein expression. The expression polymorphism of some of these proteins has potential adaptive significance.

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Domaines

Génétique

Dates et versions

hal-02665815 , version 1 (31-05-2020)

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Citer

Cristina-Maria Valcu, Céline C. Lalanne, Gerhard Müller-Starck, Christophe Plomion, Katja Schlink. Protein polymorphism between two Picea abies ecotypes as revealed by two-dimensional gel electrophoresis and tandem mass spectrometry. Journal of Heredity, 2008, 99 (4), pp.364-375. ⟨10.1093/jhered/esn007⟩. ⟨hal-02665815⟩

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