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Gene knockdown by RNAi in the pea aphid Acyrthosiphon pisum

Abstract : RNA interference (RNAi) is a powerful method to inhibit gene expression in a sequence specific manner. Here, we described the development of RNAi by micro-injection of double-stranded RNA ( dsRNA) in the pea aphid Acyrthosiphon pisum. Injection of dsRNA into whole aphid body induced the silencing of two marker genes with different expression patterns: the ubiquitously expressed Ap-crt genes encoding a calreticulin and the gut specific Ap-cath-L gene encoding a cathepsin-L. Time-course analysis of the silencing showed similar temporal patterns for both genes: inhibition started at 1 day after injection, reached its maximum at 5 days and stopped at 7 days. A comparable 40% decrease of gene expression was observed for Ap-crt and Ap-cath-L. The pea aphid is the first Hemipteran insect for which genome sequence will be available soon. The gene knockdown technique developed in this study will be an essential post-genomic tool for further investigations in aphidology.
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Stéphanie Jaubert, Gaël Le Trionnaire, Joel Bonhomme, G.K. Christophides, Claude Rispe, et al.. Gene knockdown by RNAi in the pea aphid Acyrthosiphon pisum. BMC Biotechnology, BioMed Central, 2007, 7 (63), n.p. ⟨10.1186/1472-6750-7-63⟩. ⟨hal-02666126⟩

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