Role of plant residues in determining temporal patterns of the activity, size and structure of nitrate reducer communities in soil - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement
Article Dans Une Revue Applied and Environmental Microbiology Année : 2010

Role of plant residues in determining temporal patterns of the activity, size and structure of nitrate reducer communities in soil

Dominique Chèneby
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  • PersonId : 1203114
David Bru
  • Fonction : Auteur
  • PersonId : 1144376
  • IdHAL : david-bru
Noemie Pascault
  • Fonction : Auteur
Pierre-Alain Maron
Lionel Ranjard

Résumé

The incorporation of plant residues into soil not only represents an opportunity to limit soil organic matter depletion resulting from cultivation but also provides a valuable source of nutrients such as nitrogen. However, the consequences of plant residue addition on soil microbial communities involved in biochemical cycles other than the carbon cycle are poorly understood. In this study, we investigated the responses of one N-cycling microbial community, the nitrate reducers, to wheat, rape, and alfalfa residues for 11 months after incorporation into soil in a field experiment. A 20- to 27-fold increase in potential nitrate reduction activity was observed for residue-amended plots compared to the nonamended plots during the first week. This stimulating effect of residues on the activity of the nitrate-reducing community rapidly decreased but remained significant over 11 months. During this period, our results suggest that the potential nitrate reduction activity was regulated by both carbon availability and temperature. The presence of residues also had a significant effect on the abundance of nitrate reducers estimated by quantitative PCR of the narG and napA genes, encoding the membrane-bound and periplasmic nitrate reductases, respectively. In contrast, the incorporation of the plant residues into soil had little impact on the structure of the narG and napA nitrate-reducing community determined by PCR-restriction fragment length polymorphism (RFLP) fingerprinting. Overall, our results revealed that the addition of plant residues can lead to important long-term changes in the activity and size of a microbial community involved in N cycling but with limited effects of the type of plant residue itself.

Dates et versions

hal-02666704 , version 1 (31-05-2020)

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Citer

Dominique Chèneby, David Bru, Noemie Pascault, Pierre-Alain Maron, Lionel Ranjard, et al.. Role of plant residues in determining temporal patterns of the activity, size and structure of nitrate reducer communities in soil. Applied and Environmental Microbiology, 2010, 76 (21), pp.7136-7143. ⟨10.1128/AEM.01497-10⟩. ⟨hal-02666704⟩
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