Accéder directement au contenu Accéder directement à la navigation
Article dans une revue

Per os administered refined olive oil and marine PUFA-rich oils reach the cornea: possible role on oxidative stress through caveolin-1 modulation

Abstract : Olive oil and fish oils are known to possess beneficial properties for human health. We investigated whether different oils and fatty acids alone were able to decrease oxidative stress induced on corneal cells. In our in vivo study, rats were fed with marine oils rich in polyunsaturated fatty acids (PUFA) or refined olive oil during 28 days. At the end of the protocol, corneas were analysed for their fatty acids composition to study the incorporation of fatty acids in cell membranes. In our in vitro study, a human corneal cell line was incubated with marine oils or refined olive oil and subjected to oxidative stress (tBHP 50 muM, 1 hour). Effects on reactive oxygen species generation, mitochondria and caveolin-1 expression were studied using microcytofluorometry, flow cytometry and confocal microscopy. Our results indicate that dietary oils changed the fatty acids composition of corneal cell membranes. According to our results, PUFA-rich oils and refined olive oil (free of antioxidants) blocked reactive oxygen species production. Oleic acid, the major fatty acid of olive oil, also decreased oxidative stress. Moreover, oleic acid modified caveolin-1 expression. Antioxidant properties of oleic acid could be due to disruption of membrane microdomains such as caveolae. Oleic acid, a potential potent modulator of oxidative stress, could be added to PUFA-rich oils to prevent oxidative stress-linked corneal pathology.
Type de document :
Article dans une revue
Liste complète des métadonnées

https://hal.inrae.fr/hal-02668144
Déposant : Migration Prodinra <>
Soumis le : dimanche 31 mai 2020 - 12:11:13
Dernière modification le : mercredi 14 octobre 2020 - 04:08:39

Fichier

2009_Dutot_Nutrition_1
Fichiers éditeurs autorisés sur une archive ouverte

Identifiants

Collections

Citation

Melody Dutot, Hong Liang, Chantal Martin, Delphine Rousseau, Alain Grynberg, et al.. Per os administered refined olive oil and marine PUFA-rich oils reach the cornea: possible role on oxidative stress through caveolin-1 modulation. Nutrition and Metabolism, BioMed Central, 2009, 6, pp.1-9. ⟨10.1186/1743-7075-6-48⟩. ⟨hal-02668144⟩

Partager

Métriques

Consultations de la notice

37

Téléchargements de fichiers

70