New insights into the Enterococcus faecalis CroRS two-component system obtained using a differential-display random arbitrarily primed PCR approach
Résumé
Using a modified random arbitrarily primed PCR approach, the operon encoding the Enterococcus faecalis JH2-2 CroRS two-component regulatory system was shown to be repressed during stationary phase, and a CroRS-regulated operon (glnQHMP) was identified. Gel retardation assays showed that the CroR regulator binds specifically to the glnQHMP promoter.