Skip to Main content Skip to Navigation
Journal articles

Refined solution structure of a liganded type 2 wheat nonspecific lipid transfer protein

Abstract : The refined structure of a wheat type 2 nonspecific lipid transfer protein (ns-LTP2) liganded withl-α-palmitoylphosphatidylglycerol has been determined by NMR. The 15N-labeled protein was produced in Pichia pastoris. Physicochemical conditions and ligandation were intensively screened to obtain the best NMR spectra quality. This ns-LTP2 is a 67-residue globular protein with a diameter of about 30 Å. The structure is composed of five helices forming a right superhelix. The protein presents an inner cavity, which has been measured at 341 Å3. All of the helices display hydrophobic side chains oriented toward the cavity. The phospholipid is found in this cavity. Its fatty acid chain is completely inserted in the protein, the l-α-palmitoylphosphatidylglycerol glycerol moiety being located on a positively charged pocket on the surface of the protein. The superhelix structure of the protein is coiled around the fatty acid chain. The overall structure shows similarities with ns-LTP1. Nevertheless, large three-dimensional structural discrepancies are observed for the H3 and H4 α-helices, the C-terminal region, and the last turn of the H2 helix. The lipid is orthogonal to the orientation observed in ns-LTP1. The volume of the hydrophobic cavity appears to be in the same range as the one of ns-LTP1, despite the fact that ns-LTP2 is shorter by 24 residues.
Document type :
Journal articles
Complete list of metadata

Cited literature [59 references]  Display  Hide  Download

https://hal.inrae.fr/hal-02675602
Contributor : Migration Prodinra <>
Submitted on : Sunday, May 31, 2020 - 5:48:49 PM
Last modification on : Tuesday, April 6, 2021 - 10:56:02 AM

File

71076_20101105031800161_1.pdf
Publisher files allowed on an open archive

Identifiers

Collections

Citation

Jean-Luc Pons, Frédéric de Lamotte, Marie Francoise Gautier, Marc-André Delsuc. Refined solution structure of a liganded type 2 wheat nonspecific lipid transfer protein. Journal of Biological Chemistry, American Society for Biochemistry and Molecular Biology, 2003, 278 (16), pp.14249-14256. ⟨10.1074/jbc.M211683200⟩. ⟨hal-02675602⟩

Share

Metrics

Record views

81

Files downloads

113