Quantitative 31P NMR spectroscopy of lignins from transgenic poplars - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement Access content directly
Journal Articles Holzforschung Year : 2001

Quantitative 31P NMR spectroscopy of lignins from transgenic poplars

Abstract

Milled wood lignins isolated from genetically modified poplar trees were studied by quantitative 31P NMR spectroscopy, in combination with thioacidolysis and mild alkaline hydrolysis. The genetic transformation of the trees included down regulation of CAD and COMT enzyme activities. These analyses confirmed that moderate CAD down-regulation does not substantially alter lignin structure. In contrast, severe CAD deficiency alters the lignin structure more profoundly by decreasing the syringyl/guaiacyl ratio and increasing the degree of condensation of the lignin. The most severe alterations were observed in the lignins from the COMT-transformed lines.

Dates and versions

hal-02677943 , version 1 (31-05-2020)

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Leonid G. Akim, Dimitris S. Argyropoulos, Lise L. Jouanin, Jean-Charles Leplé, Gilles G. Pilate, et al.. Quantitative 31P NMR spectroscopy of lignins from transgenic poplars. Holzforschung, 2001, 55 (4), pp.386-390. ⟨10.1515/HF.2001.064⟩. ⟨hal-02677943⟩
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