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Fusion to a carrier protein and a synthetic propeptide enhances E7 HPV-16 production and secretion in Lactococcus lactis

Abstract : An inducible system to improve and stabilize the production of an extremely labile protein (E7 antigen of human papillomavirus type 16) was developed in the food-grade bacterium Lactococcus lactis. A protein carrier, the staphylococcal nuclease Nuc, was fused either to N- or C-termini of E7 protein, and the resulting hybrid proteins were rescued from intracellular proteolysis but poorly secreted by L. lactis. A synthetic propeptide (LEISSTCDA) was then fused and significantly improved the secretion efficiency of the hybrid protein Nuc-E7 by L. lactis.
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https://hal.inrae.fr/hal-02678476
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Submitted on : Sunday, May 31, 2020 - 8:29:41 PM
Last modification on : Thursday, September 23, 2021 - 4:28:03 PM

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Luis Bermudez Humaran, Naima Cortes-Perez, Yves Le Loir, Alexandra Gruss, Cristina Rodríguez-Padilla, et al.. Fusion to a carrier protein and a synthetic propeptide enhances E7 HPV-16 production and secretion in Lactococcus lactis. Biotechnology Progress, Wiley, 2003, 19 (3), pp.1101-1104. ⟨10.1021/bp0340077⟩. ⟨hal-02678476⟩

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