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Influence of cooling temperature and duration on cold adaptation of Lactobacillus acidophilus RD758

Abstract : The effect of different cooling temperatures and durations on resistance to freezing and to frozen storage at −20 °C in Lactobacillus acidophilus RD758 was studied, by using a central composite rotatable design. A cold adaptation was observed when the cells were maintained at moderate temperature (26 °C) for a long time (8 h) before being cooled to the final temperature of 15 °C. These conditions led to a low rate of loss in acidification activity during frozen storage (0.64 min day−1) and a high residual acidification activity after 180 days of frozen storage (1011 min). The experimental design allowed us to determine optimal cooling conditions, which were established at 28 °C during 8 h. Adaptation to cold temperatures was related to an increase in the unsaturated to saturated fatty acid ratio and in the relative cycC19:0 fatty acid concentration. Moreover, an increased synthesis of four specific proteins was observed as an adaptive response to the optimal cooling conditions. They included the stress protein ATP-dependent ClpP and two cold induced proteins: pyruvate kinase and a putative glycoprotein endopeptidase.
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https://hal.inrae.fr/hal-02679137
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Submitted on : Sunday, May 31, 2020 - 9:10:53 PM
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Yu Wang, Jérôme J. Delettre, Alain A. Guillot, Georges G. Corrieu, Catherine Béal. Influence of cooling temperature and duration on cold adaptation of Lactobacillus acidophilus RD758. Cryobiology, Elsevier, 2005, 50 (3), pp.294-307. ⟨10.1016/j.cryobiol.2005.03.001⟩. ⟨hal-02679137⟩

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