Isolation and characterization of efficient isoxaben-transforming Microbacterium sp strains from four European soils - Archive ouverte HAL Access content directly
Journal Articles Pest Management Science Year : 2002

Isolation and characterization of efficient isoxaben-transforming Microbacterium sp strains from four European soils

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Abstract

Nutrient-agar plates containing isoxaben (500 mg litre(-1)) were used to isolate isoxaben-metabolising bacteria from four European soils incubated with the herbicide under laboratory conditions. In flask experiments, inoculation of a basal salts medium containing nitrogen and [phenyl-U-C-14]isoxaben with an isolate (B2b) resulted in 33% recovery of the initial radioactivity as [C-14]carbon dioxide after 2 weeks. A major metabolite identified by GC-MS and NMR analysis as 3-(1-ethyl-1-methylpropyl)isoxazol-5-ylamine accumulated both in basal salts and nutrient broth media. 2,6-Dimethoxybenzoic acid, a suspected metabolite of isoxaben, was not detected in either liquid media. However, the capability of the B2b isolate to use 2,6-dimethoxybenzoic acid as a source of carbon was demonstrated. Soil inoculation with the B2b strain resulted in an increase in the recovery of [C-14]carbon dioxide from both [phenyl-U-C-14] and [isoxazole-(5)-C-14]isoxaben. The metabolite identified as 3-(1-ethyl-1-methylpropyl)isoxazole-5-ylamine only accumulated if the soil was autoclaved before inoculation. This metabolite was rapidly mineralized by the microflora of a natural soil without history of isoxaben treatment. Homology patterns of sequenced 16S rDNA between isoxaben-transforming isolates and reference strains showed that the four isolates identified belonged to the genus Microbacterium.
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hal-02679849 , version 1 (19-01-2023)

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Attribution - ShareAlike - CC BY 4.0

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Sandra Arrault, Stephane Desaint, Colette Catroux, Etienne Semon, Christian Mougin, et al.. Isolation and characterization of efficient isoxaben-transforming Microbacterium sp strains from four European soils. Pest Management Science, 2002, 58, pp.1229-1235. ⟨10.1002/ps.599⟩. ⟨hal-02679849⟩
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