Skip to Main content Skip to Navigation
Journal articles

T-DNA transfer from Agrobacterium tumefaciens to the ectomycorrhizal fungus Pisolithus microcarpus

Abstract : The model ectomycorrhizal fungus Pisolithus microcarpus isolate 441 was transformed by using Agrobacterium. tumefaciens LBA1100 and AGL-1. The selection marker was the Shble gene of Streptoallotecius hidustanus, conferring resistance to phleomycin, under the control of the gpd gene promoter and terminator of Schizophyllum commune. Transformation resulted in phleomycin resistant clones which were confirmed by PCR to contain the resistance cassette. A. tumefaciens-mediated gene transfer would allow the development of RNA interference technology in P. microcarpus.
Document type :
Journal articles
Complete list of metadata

Cited literature [24 references]  Display  Hide  Download

https://hal.inrae.fr/hal-02681826
Contributor : Migration Prodinra <>
Submitted on : Sunday, May 31, 2020 - 11:55:38 PM
Last modification on : Sunday, April 11, 2021 - 6:58:02 PM

File

10623_20110411023640531_1.pdf
Publisher files allowed on an open archive

Identifiers

  • HAL Id : hal-02681826, version 1
  • PRODINRA : 10623

Collections

Citation

A.G. Pardo, Minna Kemppainen, D. Valdemoros, Sébastien Duplessis, Francis Martin, et al.. T-DNA transfer from Agrobacterium tumefaciens to the ectomycorrhizal fungus Pisolithus microcarpus. Revista Argentina de Microbiología, Asociación Argentina de Microbiología, 2005, 37, pp.69-72. ⟨hal-02681826⟩

Share

Metrics

Record views

15

Files downloads

16