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Quantitative detection of Listeria monocytogenes in biofilms by real-time PCR

Abstract : A quantitative method based on a real-time PCR assay to enumerate Listeria monocytogenes in biofilms was developed. The specificity for L. monocytogenes of primers targeting the listeriolysin gene was demonstrated using a SYBR Green I real-time PCR assay. The number of L. monocytogenes detected growing in biofilms was 6 x 10(2) CFU/cm2.
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https://hal.inrae.fr/hal-02682519
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Submitted on : Monday, June 1, 2020 - 1:16:57 AM
Last modification on : Monday, July 19, 2021 - 5:52:03 PM

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Morgan Guilbaud, Pierre de Coppet, Fabrice Bourion, Cinta Rachman, Herve Prévost, et al.. Quantitative detection of Listeria monocytogenes in biofilms by real-time PCR. Applied and Environmental Microbiology, American Society for Microbiology, 2005, 71 (4), pp.2190-2194. ⟨10.1128/AEM.71.4.2190-2194.2005⟩. ⟨hal-02682519⟩

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