Quantitative detection of Listeria monocytogenes in biofilms by real-time PCR - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement Access content directly
Journal Articles Applied and Environmental Microbiology Year : 2005

Quantitative detection of Listeria monocytogenes in biofilms by real-time PCR

Abstract

A quantitative method based on a real-time PCR assay to enumerate Listeria monocytogenes in biofilms was developed. The specificity for L. monocytogenes of primers targeting the listeriolysin gene was demonstrated using a SYBR Green I real-time PCR assay. The number of L. monocytogenes detected growing in biofilms was 6 x 10(2) CFU/cm2.

Dates and versions

hal-02682519 , version 1 (01-06-2020)

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Morgan Guilbaud, Pierre de Coppet, Fabrice Bourion, Cinta Rachman, Herve H. Prévost, et al.. Quantitative detection of Listeria monocytogenes in biofilms by real-time PCR. Applied and Environmental Microbiology, 2005, 71 (4), pp.2190-2194. ⟨10.1128/AEM.71.4.2190-2194.2005⟩. ⟨hal-02682519⟩
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