Random mtDNA deletions and functional consequence in aged human skeletal muscle - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement Access content directly
Journal Articles Biochemical and Biophysical Research Communications Year : 2005

Random mtDNA deletions and functional consequence in aged human skeletal muscle

Abstract

Mitochondrial respiratory chain deteriorates with age, mostly in tissues with high energy requirements. Damage to mitochondrial DNA (mtDNA) by reactive oxygen species is thought to contribute primarily to this impairment. However, the overall extent of random mtDNA mutations has still not been evaluated. We carried out molecular and biochemical analyses in muscle biopsies from healthy young and aged subjects. Deleted mtDNA accumulation was followed by both quantitative PCR analysis to quantify total mtDNA, and Southern-blotting, to determine deleted to full length mtDNA ratio. Enzymatic activities of the mitochondrial respiratory chain were measured in all subjects. Randomly deleted mtDNA appeared mainly in the oldest subjects (beyond 80 years old), affecting up to 70% of mtDNA molecules. The activities of complexes III and IV of the respiratory chain, complexes with mtDNA encoded subunits, are lower in the aged subjects. Physical activity could be one major parameter modulating the mitochondrial respiratory chain activity in aged muscle.

Dates and versions

hal-02682861 , version 1 (01-06-2020)

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Béatrice Chabi, Bénédicte de Camaret, Arnaud Chevrollier, Stéphane Boisgard, Georges Stepien. Random mtDNA deletions and functional consequence in aged human skeletal muscle. Biochemical and Biophysical Research Communications, 2005, 332 (2), pp.542-549. ⟨10.1016/j.bbrc.2005.04.153⟩. ⟨hal-02682861⟩
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