Skip to Main content Skip to Navigation
Journal articles

Batch cultures of recombinant Lactococcus lactis subsp. lactis in a stirred fermentor. II. Plasmid transfer in mixed cultures

Résumé : The transfer of plasmids was studied in a stirred fermentor in the course of mixed batch cultures combining recombinant strains of Lactococcus lactis subsp. lactis (donor strains) with L. lactis subsp. lactis CNRZ 268M3 (recipient strain). Donor strains contained one or two of the following plasmids (coding for erythromycin or chloramphenicol resistance): pIL205 (self-transmissible), pIL252, pIL253 (non-transmissible but mobilizable by pIL205, respectively small and large copy number) and pE194 (inserted in the chromosome). Only self-transmissible plasmid pIL205 was transferred, with frequencies ranging from 10–7 to 10–8 after 12 h of fermentation. These frequencies were 60–400 times lower than in unstirred M17 broth and 100 000 times lower than on agar medium. In the latter case, non-transmissible plasmids pIL252 and pIL253 were mobilized by pIL205 with a frequency of about 10–5–10–6.
Document type :
Journal articles
Complete list of metadata

https://hal.inrae.fr/hal-02703027
Contributor : Migration Prodinra <>
Submitted on : Monday, June 1, 2020 - 2:09:23 PM
Last modification on : Friday, September 17, 2021 - 1:59:06 PM

Identifiers

  • HAL Id : hal-02703027, version 1
  • PRODINRA : 99706

Collections

Citation

N. El Alami, Clair-Yves Boquien, Georges Corrieu. Batch cultures of recombinant Lactococcus lactis subsp. lactis in a stirred fermentor. II. Plasmid transfer in mixed cultures. Applied Microbiology and Biotechnology, Springer Verlag, 1992, 37 (3), pp.364-368. ⟨hal-02703027⟩

Share

Metrics

Record views

18