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Journal articles
Effect of maturation duration on desiccation tolerance in hybrid larch (Larix leptoeuropaea Dengler) somatic embryos
Abstract : Cotyledonary somatic embryos ofLarix × leptoeuropaea that developed after various maturation times on media containing abscisic acid showed different frequencies of conversion into plants. Drying of these somatic embryos under high relative humidity (RH) before germination improved plantlet recovery and eliminated differences in the performance of somatic embryos matured for different times. However, dehydration of somatic embryos under 98% RH to a water content below that of zygotic embryos excised from mature seeds (0.97 and 1.36 g H2O/g dry weight, respectively) showed a strong positive correlation between longer maturation time and desiccation tolerance. Drying somatic embryos at 4° C under 59% RH for 1 wk resulted in desiccation to a water content of 0.30 g H2O/g dry weight, which was the closest to the hydration state of zygotic embryos in dried, stored seeds (0.20 g H2O/g dry weight). Under this condition, only somatic embryos matured for 5 wk germinated and produced plantlets at a relatively high frequency (73 and 41%, respectively).
https://hal.inrae.fr/hal-02713452
Contributor : Migration Prodinra Connect in order to contact the contributor
Submitted on : Monday, June 1, 2020 - 6:58:06 PM
Last modification on : Thursday, March 31, 2022 - 4:22:50 AM
Contributor : Migration Prodinra Connect in order to contact the contributor
Submitted on : Monday, June 1, 2020 - 6:58:06 PM
Last modification on : Thursday, March 31, 2022 - 4:22:50 AM
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