An immunodominant cytotoxic T cell epitope on the VP7 rotavirus protein overlaps the H2 signal peptide - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement Access content directly
Journal Articles Journal of General Virology Year : 1993

An immunodominant cytotoxic T cell epitope on the VP7 rotavirus protein overlaps the H2 signal peptide

Abstract

C57BL/6 (H-2b) mice were primed with the bovine RF strain of rotavirus to study the induction of CD8+ cytotoxic T lymphocytes (CTLs). These rotavirus-specific CTLs were detected only after in vitro restimulation with the virus. Using a recombinant vaccinia virus we identified the RF VP7 protein as a major target of these CTLs. The response against this protein was obtained also after in vitro restimulation with simian SA11 and human WA strains of rotavirus. Using published Db and Kb allele-specific motifs to predict possible CTL epitopes in the RF VP7 protein, we synthesized and tested 18 predicted peptides of VP7. Only one peptide was able to sensitize target cells at a concentration below 5 x 10(-7) M. This CTL epitope was also induced by immunization with the RF VP7 expressed with a baculovirus vector, and was shown to be immunodominant by its capacity to inhibit, in an unlabelled target assay, the bulk response against cells infected with recombinant vaccinia virus expressing VP7. This CTL epitope overlaps the H2 signal peptide of the protein.

Domains

Virology
No file

Dates and versions

hal-02713554 , version 1 (01-06-2020)

Identifiers

Cite

Manuel A. Franco, Isidro Prieto, Marie Labbe, Didier Poncet, Francisco Borràs-Cuesta, et al.. An immunodominant cytotoxic T cell epitope on the VP7 rotavirus protein overlaps the H2 signal peptide. Journal of General Virology, 1993, 74, pp.2579-2586. ⟨hal-02713554⟩
6 View
0 Download

Altmetric

Share

Gmail Facebook X LinkedIn More