Plazmidnye invertirovannye povtory obespechivaiut duplikatsiiu ili tochnoe vyrezanie vstroennykh v nikh fragmentov DNK. - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement
Article Dans Une Revue Molekuliarnaia genetika, mikrobiologiia i virusologiia Année : 1988

[Plasmid inverted repeats provide for duplication or precise excision of DNA inserts].

Plazmidnye invertirovannye povtory obespechivaiut duplikatsiiu ili tochnoe vyrezanie vstroennykh v nikh fragmentov DNK.

Résumé

A plasmid containing inverted repeats is constructed in Bacillus subtilis. Insertion of DNA fragments into the plasmid inverted repeats results either in the precise excision of the insert or in its duplication in the opposite inverted repeat. These rearrangements are due to the presence of inverted repeats only. Two recombination events are possibly responsible for these phenomena. During the first step of the recombination two plasmid monomers form a dimer molecule. During the second step the intramolecular recombination between the direct repeats in the dimer structure leads to the formation of two rearranged plasmid monomers devoid of insertion or containing two DNA inserts. Proposed dimeric intermediate is unstable in B. subtilis. However, it is isolated from Escherichia coli recA, cells. Plasmids containing the inverted repeats can serve as a model to study plasmid recombination in B. subtilis cells.
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Dates et versions

hal-02725539 , version 1 (02-06-2020)

Identifiants

Citer

Vladimir E Bidnenko, M Ia Khaĭkinson. Plazmidnye invertirovannye povtory obespechivaiut duplikatsiiu ili tochnoe vyrezanie vstroennykh v nikh fragmentov DNK.. Molekuliarnaia genetika, mikrobiologiia i virusologiia, 1988, 3, pp.27-33. ⟨hal-02725539⟩
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