The branched orobanche, Phelipanche ramosa L., is an obligate parasitic weed that has become, in France, a major agronomic problem in tobacco crops; yield and quality losses can be total and cause growers to reduce the producing area due to lack of available healthy plots or to abandon the crop. Because chemical management is not effective, biocontrol is certainly the alternative to propose but there is currently no biological control agent on the market to meet the expectations of producers and control this parasitic plant. Only a few microorganisms or molecules, are presented as potential and promising candidates in conclusion of scientific articles but the finalization is absent. A collection of 525 fungal isolates was made from 400 symptomatic orobanches taken from 28 plots representative of the tobacco growing regions in France to identify virulent fungi, adapted to the local pedoclimatic conditions and usable to control locally the development of orobanches. Indeed, pathogenicity tests, conducted in controlled bioassays and taxonomic identification based on morphometric and molecular criteria (ITS and EF1-alpha barcoding) are partially performed to date but analysing the distribution of isolates associated to symptomatic plants already reveals a link between the geographical origin of the isolates and their taxon of belonging. This first result argues for the identification of locally adapted biocontrol agents. Of the taxa already identified, Fusarium, Rhizoctonia, Alternaria, Epicoccum, Trichoderma, the genus Fusarium is the most represented and includes the species F. solani and F. oxysporum. The specificity of the interaction between the selected isolates and the orobanches, and more particularly Phelipanche ramosa, should be finely characterized to avoid that the potential biological control agents affect other plants than the targets.