Skip to Main content Skip to Navigation
Conference papers

Physical events during cryopreservation: consequences on cells’ post-thaw performance and on cryobiological protocols optimisation

Abstract : For decades, scientists have looked for the causes of cell cryoinjury so as to identify the optimum conditions for freezing and storing frozen cells. Cell dehydration and intracellular ice formation have been argued as the main mechanisms of cell damage. Maintaining the cells in a vitreous matrix below the glass transition temperature of the surrounding medium is known as a key condition for cell preservation. However, it is only in 2013 that the measurement of the intracellular glass transition of different cell types has been made possible. Moreover, the relevance of intracellular and extracellular vitrification during slow cooling of microorganisms and mammalian cells has been the subject of recent research. The physical events taking place during freezing are reviewed here, focusing on the role of the physical state of the intracellular and extracellular environments in determining the response of cells to stresses encountered during cryopreservation. The implications on cryoprotectants selection, freezing rates, and controlled cooling endpoint to be set for cell storage are discussed.
Complete list of metadata

https://hal.inrae.fr/hal-02734376
Contributor : Migration Prodinra Connect in order to contact the contributor
Submitted on : Tuesday, November 10, 2020 - 11:40:00 AM
Last modification on : Thursday, July 22, 2021 - 10:04:10 AM

File

6_ISLFD-2019_FFonseca_abstract...
Files produced by the author(s)

Identifiers

  • HAL Id : hal-02734376, version 1
  • PRODINRA : 480829

Collections

Citation

Fernanda Fonseca, Julie Meneghel, Peter Kilbride, Stéphanie Passot, G. John Morris. Physical events during cryopreservation: consequences on cells’ post-thaw performance and on cryobiological protocols optimisation. 9. International Symposium on Lyophilization of Pharmaceuticals ISLFD, Sep 2019, Ghent, Belgium. ⟨hal-02734376⟩

Share

Metrics

Record views

73

Files downloads

13