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Proteomics analysis of albumins and globulins from either endosperm or the aleurone layer

Abstract : To illustrate the usefulness of the proteomics tool, we report on two experiments whose objectives were to (1) analyse albumins and globulins in developing kernel, or (2) map albumins and globulins using segregating progeny, or reveal these proteins in the aleurone layer of two different cultivar. Proteomic analysis every 50°C day (average cumulative day temperature) during kernel formation from the ovule stage to 18 days after anthesis allowed many albumins and globulins to be tracked during cell and tissue formation. Quantitative analysis of spot volume and qualitative analysis (appearance/disappearance of a spot) evidenced five types of protein expression. Two-dimensional electrophoresis pH 4-7 x SDS Page of the albumins-globulins extracted from wholemeal kernels of ‘Synthetic’ or ‘Opata’ revealed 2125 and 2163 spots respectively. Only 10.6% of the spots differed between these two parents of the mapped progeny. The unbiased segregating spots were successfully mapped on 21 chromosomes. Wheat AL was isolated from mature seeds of cv. Chinese Spring and cv. Recital and more than 700 Coomassie stained proteins were revealed on 24 cm IPGE pH 3-10 x SDS-Page. Nearly 20% of these spots differed between the two cultivars. Proteomics analysis of seed is an increasingly available tool that enables biochemists and geneticists to investigate the diversity and function of the proteins expressed in different cell organites such as protein bodies, starch granules or kernel compartments
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  • HAL Id : hal-02753313, version 1
  • PRODINRA : 468469

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Gerard Branlard, Marielle Merlino, Isabelle Nadaud, Emmanuelle Bancel, Bastien Laubin. Proteomics analysis of albumins and globulins from either endosperm or the aleurone layer. 1. American Latin conference ICC Conference, Sep 2007, Rosario, Argentina. ⟨hal-02753313⟩

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