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Identification and quantification of phosphorus in cheeses - methodological investigations by solid-state 31P NMR spectroscopy

Abstract : NMR has been shown to be an interesting tool for investigating dairy foods, in particular cheese products, for which the method is non-destructive and well suited for investigating their compounds showing different structure and mobility during ripening. Solid-state magic angle spinning 31P NMR spectroscopy was used to characterize the structure and composition of semi-hard cheeses. Assignment including overlapping resonances from mobile/immobile phosphorylated serine residues and inorganic calcium phosphates, has been realised using different NMR techniques (spin echo, cross polarisation and single pulse excitation) and by comparison with spectra of literature. Studies at various ripening stages and salt contents have been achieved in order to understand the impact of salt on the phosphates structures and mobility within the cheese matrix, as well as its influence in enzymatic activities.
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https://hal.inrae.fr/hal-02756831
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Submitted on : Wednesday, June 3, 2020 - 11:26:27 PM
Last modification on : Thursday, August 5, 2021 - 4:30:02 PM

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  • HAL Id : hal-02756831, version 1
  • PRODINRA : 31798

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Corinne Rondeau-Mouro, Mallory Gobet, Bernard Mietton, S Buchin, Céline Moreau. Identification and quantification of phosphorus in cheeses - methodological investigations by solid-state 31P NMR spectroscopy. 9th International Conference on the Applications of Magnetic Resonance in Food Science "Challenges in a changing world", September 15th -17th 2008, Sep 2008, Reykjavík, Iceland. ⟨hal-02756831⟩

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