Accéder directement au contenu Accéder directement à la navigation
Communication dans un congrès

Mechanisms by which the Flavonoid Dioclein Decreases Cytokines and Chemokines Levels in vitro

Abstract : Introduction : Dioclein is a flavonoid isolated from Dioclea grandiflora , a brazilian vine. Previous studies showed that dioclein has an important cardiovascular effect. Its possible antioxidant and anti-inflammatory effect, however, has not yet been evaluated. Here, we investigated whether dioclein exhibits such properties. Methods : C57BL/6 mice peritoneal macrophages were cultured. Cells were treated with increased concentrations of dioclein and stimulated with LPS. Supernatant was collected after 24 hours. Cytokines and chemokines levels were determined by ELISA. Nitric oxide (NO) was assessed by Griess reaction. Cells were also treated with dioclein and stimulated with zymosan. Reactive oxygen species production was evaluated by luminol-dependent chemiluminescence assay. Antioxidant effects were also evaluated using the xanthine/xanthine oxidase (X/XO) lucigenin-dependent chemiluminescence assay. Inhibition of PDE4 by dioclein was evaluated by a binding assay. Results : Dioclein decreased LPS-induced production of NO, TNF-a and IL-6 in a concentration-dependent manner. Production of chemokines CXCL1-3/KC and CCL2/JE were also decreased. Dioclein were antioxidant, as evaluated by luminol-induced chemiluminescence assay and by X/XO system, similar to well-known positive controls (BHT and a-ocopherol). PDE4 was inhibited at concentrations similar to those resembling anti-inflammatory and antioxidant effects. Discussion: Dioclein may exhibit an anti-inflammatory activity trough a combined antioxidant and PDE4-inhibition roperty. These two major mechanisms could justify the ability of the flavonoid in decrease cytokines and chemokines levels.
Type de document :
Communication dans un congrès
Liste complète des métadonnées
Déposant : Migration Prodinra <>
Soumis le : jeudi 4 juin 2020 - 00:21:30
Dernière modification le : vendredi 12 juin 2020 - 10:43:26


  • HAL Id : hal-02757357, version 1
  • PRODINRA : 274903



Rodrigo Guabiraba, Ana Lucia Campanha, Adriano L.S. Souza, Helton C. Santiago, Virginia S. Lemos, et al.. Mechanisms by which the Flavonoid Dioclein Decreases Cytokines and Chemokines Levels in vitro. 38. Congresso Brasileiro de Farmacologia e Terapêutica Experimental, Oct 2006, Ribeirão Preto, Brazil. ⟨hal-02757357⟩



Consultations de la notice