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Communication dans un congrès

Characterization of transgenic apples expressing the HrpN gene

Abstract : Fire blight is the major bacterial disease of Maloideae caused by the necrogenic bacterium Erwinia amylovora, which pathogenicity requires a functional type III protein secretion system (TTSS). The protein harpin NEa is secreted through the TTSS and targeted to the intercellular spaces of plant tissues where it acts as an elicitor of plant defense reactions. In order to create novel mechanisms for fire blight resistance in apple, we have generated transgenic apples expressing the hrpN gene from E. amylovora under the control of the constitutive CaMV35S or the pathogen-inducible tobacco Str246C promoters, with or without the tobacco PR1 gene secretion signal peptide. The characterization of these harpin NEa transgenic apples was carried out by: (1) quantification of the constitutive and induced expression of the transgene (by Q-RT-PCR); (2) in planta localization of the harpin NEa protein; (3) evaluation of fire blight resistance after greenhouse inoculation; (4) targeted transcriptomic analysis to identify defenses induced by the presence of the transgene.
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Communication dans un congrès
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Déposant : Migration Prodinra <>
Soumis le : jeudi 4 juin 2020 - 02:20:29
Dernière modification le : vendredi 12 juin 2020 - 10:43:26


  • HAL Id : hal-02757712, version 1
  • PRODINRA : 30396
  • WOS : 000305708800087



Emilie Vergne, Sophie Sourice, Fabrice Dupuis, Karine Loridon, Thomas Dugé de Bernonville, et al.. Characterization of transgenic apples expressing the HrpN gene. 1. International Symposium on Biotechnology of Fruit Species : Biotechfruit 2008, Sep 2008, Dresden, Germany. ⟨hal-02757712⟩



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