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Communication dans un congrès

Use of PCAH as a molecular marker for estimating the diversity of the protocatechuate-degrading bacterial community in soil environment

Abstract : Microorganisms degrading phenolic compounds play an important role in soil carbon cycling. The pcaH gene encoding a key ring-cleaving enzyme of the β -ketoadipate pathway was selected as a functional marker. Using a degenerate primer pair, pcaH fragments were cloned from two soils. The RFLP screening of 150 pcaH clones yielded 68 RFLP families. Comparison of 86 deduced amino acid sequences displayed 70 % identity to known PcaH sequences. Phylogenetic analysis results in two major groups mainly related to PcaH sequences from Actinobacteria and Proteobacteria phyla. This gene constitutes a suitable molecular marker to study the diversity of this functional group.
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Communication dans un congrès
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https://hal.inrae.fr/hal-02758489
Déposant : Migration Prodinra <>
Soumis le : jeudi 4 juin 2020 - 03:45:17
Dernière modification le : mardi 8 septembre 2020 - 17:34:05

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  • HAL Id : hal-02758489, version 1
  • PRODINRA : 51448

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Najoi El Azhari, Sylvaine Chabaud, Anthony Percept, David Bru, Fabrice Martin-Laurent. Use of PCAH as a molecular marker for estimating the diversity of the protocatechuate-degrading bacterial community in soil environment. Actes of the International Conference on Water Pollution in natural Porous media at different scales. Assessment of fate, impact and indicators (WAPO2), Apr 2007, Barcelone, Spain. ⟨hal-02758489⟩

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