Linkage mapping and QTL analysis for growth of young Pseudotsuga menziesii plants
Résumé
A controlled cross between two Douglas-fir trees, from Washington State, have been analysed for genetic mapping and for QTL detection. Their progeny have been measured for stem height, bud flushing, and polycyclism on the second and third year after sawing. A genetic map was drawn from segregations of AFLP markers recorded in 157 seedlings. These markers were obtained with 25 primer pairs tested in other Pinaceae species. Genetic map of the female parent consisted of 123 markers distributed in 24 linkage groups. QTLs have been detected by interval mapping for traits showing a normal distribution (stem height, stem elongation), and by a Kruskal and Wallis test for trait that did not show a normal distribution (bud flushing, polycyclism). QTLs have been detected for all investigated traits. QTLs for growth and flushing traits did not overlapped on the female genetic map. QTLs for vegetative flushing stage of terminal bud recorded in 2001 and for day of bud elongation recorded in 2002 did not overlapped. Further evaluations of quantitative traits are required to test time stability of QTLs.
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Origine : Fichiers produits par l'(les) auteur(s)
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