Skip to Main content Skip to Navigation
Conference papers

Artificial selection of rhizosphere microbiota associated to phenotypical changes in plant functions

Abstract : Artificial selection applied at community level is an important, but still growing topic at the interface of ecology and evolution. Its recent implementation to microbial communities holds appealing promises not only in terms of fundamental knowledge about selection itself and the levels at which it may be used, but also in terms of relevant applications to our societies, including bioremediation and agroecology. In this experimental evolution study, we performed an artificial selection of rhizosphere microbial communities inducing relevant phenotypic changes in plants. In total, we grew more than 2200 Brachypodium distachyon plants, consisting in ten consecutive generations of four-weeks, and inoculated with artificially selected rhizosphere microbiota originating from the previous generation. Selection was applied for increasing or decreasing plant nitrogen uptake based on leaves color nuances via a semi-automated high-throughput plant phenotyping platform. Ultimately, selected rhizosphere microbiota were also inoculated to maize, barley and wheat grown in two different soils to evaluate the transferability of our induced microbial properties. 16S rRNA gene and ITS amplicon sequencing revealed a rapid response of plants to artificial rhizosphere microbiota selection, with significant divergence and heritability of traits observed after few generations, correlating with specific changes in the structure of selected microbial pools. Transferability assay showed that properties linked to selected microbial communities depend on plant species, but consistently induced reproducible effects on Brachypodium distachyon in different soils. Artificial selection of rhizosphere microbiota altering plant phenotype is efficient, fast and reproducible, leveraging a whole new array of possibilities for shaping plant traits.
Complete list of metadata
Contributor : Migration Prodinra <>
Submitted on : Friday, June 5, 2020 - 1:27:45 AM
Last modification on : Wednesday, July 7, 2021 - 3:41:35 AM


  • HAL Id : hal-02787150, version 1
  • PRODINRA : 462781


Samuel Jacquiod, Aymé Spor, Laurent Philippot, Manuel Blouin. Artificial selection of rhizosphere microbiota associated to phenotypical changes in plant functions. 17th International Society of Microbial Ecology (ISME17), Aug 2018, Leipzig, Germany. ⟨hal-02787150⟩



Record views