Probing activation and deactivation of the BRASSINOSTEROID INSENSITIVE1 receptor kinase by immunoprecipitation - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement Access content directly
Book Sections Year : 2017

Probing activation and deactivation of the BRASSINOSTEROID INSENSITIVE1 receptor kinase by immunoprecipitation

Abstract

Brassinosteroids (BRs) are sterol-derived hormones that control plant growth and development. The BR receptor complex is encoded by the BRASSINOSTEROID INSENSITIVE1 (BRI1) and members of the SOMATIC EMBRYOGENESIS RECEPTOR KINASE family. For activation and deactivation, the BR receptor complex uses different posttranslational modifications and recruits various partner proteins. Here, we describe optimized immunoprecipitation protocols and variants for biochemical analyses of posttranslational modifications of BRI1 and of protein-protein interactions.

Dates and versions

hal-02788279 , version 1 (05-06-2020)

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Sara Martins, Grégory Vert, Yvon Jaillais. Probing activation and deactivation of the BRASSINOSTEROID INSENSITIVE1 receptor kinase by immunoprecipitation. Brassinosteroids, 1564 (Chapter 14), Editions Springer, 2017, Methods in Molecular Biology, 978-1-4939-6811-4. ⟨10.1007/978-1-4939-6813-8_14⟩. ⟨hal-02788279⟩
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