The Suppressors Of Cytokine Signaling (SOCS) and the cytokine-inducible SH2 domain containing protein (CIS) are a family of 8 intracellular proteins which have emerged as key regulators of the immune system. Little is known about tissue expression of SOCS and data in pigs are extremely scarce. In order to further study porcine SOCS, preliminary data must be collected. CIS and SOCS transcript expressions along with the stability of some reference genes were assessed in various tissues. The Beta-2-microgobulin reference gene was the most suitable and an over-expression of SOCS 1 mRNA was observed in thymus. Then, we characterized the immune response ofNewbom Pig Trachea (NPTr) cells to swine influenza H3N2 and the potential role of SOCS proteins in this response. Among SOCS only SOCS 1 appears to be over-expressed 3h post infection. Using NPTr cells and Precision Cut Lung Slices (PCLS) we also initiated the characterization of the interaction of H3N2 with respiratory mucosa in the first hours of infection. The establishment of a type I interferon response along with the mRNA expression of antiviral genes such as Mxl, Mx2 and RIG1 were observed. Furthermore, in vitro at early points of infection the influence of H3N2 on cellular signalisation was analysed. We observed a precocious activation of Mitogen Activated Protein Kinase (MAPK) pathways such as p38 and ERK 112 MAPK with a decrease 4h post-infection. These findings show the establishment of an antiviral response in NPTr cells and PCLS against H3N2 and suggest a possible regulation by SOCS 1 requiring further studies.