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Massive indexed parallel identification of transposon flanking sequences

Abstract : The large scale sequencing of insertion element flanking sequences has revolutionized reverse genetics in plant research: Insertion mutants can now simply be identified in silico by BLAST searching the resulting flanking sequence databases. The development of next-generation sequencing technologies has further facilitated the creation of flanking sequence collections derived from entire mutant populations. Here we describe a highly efficient and widely applicable method that we developed to amplify, sequence, and identify dTph1 transposon flanking sequences from a library of 1000 Petunia W138 individuals simultaneously.
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Michiel Vandenbussche, Jan Zethof, Tom Gerats. Massive indexed parallel identification of transposon flanking sequences. Plant transposable elements: methods and protocols, 1057, Humana Press, 2013, Methods in Molecular Biology, 978-1-62703-568-2; 978-1-62703-567-5. ⟨10.1007/978-1-62703-568-2_18⟩. ⟨hal-02810937⟩



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