Transgenic rootstocks expressing GFLV coat protein gene in a three years field trial ; resistance assessment, impact on GFLV diversity and exchanges between rootstock and scion - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement Access content directly
Proceedings Year : 2009

Transgenic rootstocks expressing GFLV coat protein gene in a three years field trial ; resistance assessment, impact on GFLV diversity and exchanges between rootstock and scion

Abstract

Transgenic rootstock lines expressing the full-length translatable coat protein (CP) gene of Grapevine fanleaf virus strain F13 (GFLV-F13) are under evaluation in an open-field trial. Control plants were contaminated after nematode-mediated transmission of GFLV after three years. In contrast, viral infection of non-transgenic scion (Vitis vinifera cv. Pinot Meunier) was delayed in vines grafted onto transgenic rootstocks. Sequencing of CP genes from GFLV isolates from this field, suggests that CP gene-expressing transgenic rootstocks do not influence significantly natural viral biodiversity. Transgenic-derived expression products were detected in GM rootstocks. However, their systemic movement was not detected suggesting that the scions remain truly wild-type. All experiments were coconstructed and followed within a Local Steering Committee. Thus, this field trial is a unique support for the assessment of environmental impact of genetically-modified plants (GMPs) and a survey of public perception of GMPs.
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Dates and versions

hal-02824772 , version 1 (06-06-2020)

Identifiers

  • HAL Id : hal-02824772 , version 1
  • PRODINRA : 40179

Cite

Caroline Hemmer, Emmanuelle Vigne, Valérie Goldschmidt, Veronique Komar, Aurélie Marmonier, et al.. Transgenic rootstocks expressing GFLV coat protein gene in a three years field trial ; resistance assessment, impact on GFLV diversity and exchanges between rootstock and scion. 16th Meeting of the International Council for the Study of Virus and virus-like diseases of the Grapevine (ICVG), Aug 2009, Dijon, France. Progrès Agricole et Viticole, pp.228-229, 2009, Progrès Agricole et Viticole. ⟨hal-02824772⟩
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