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Marquage et expression du genome chez le mais

Abstract : Neutral markers, such as isoenzymes and RFLPs (restriction fragment length polymorphism), have applications in various fields of genetics and breeding. Three examples are documented. Firstly, about 130 maize lines were compared using 46 RFLP probes distributed over the 10 chromosomes. Data analyses and distance indices gave a classification consistent with heterotic groups and pedigrees, showing the value of molecular markers for characterizing unknown material. Distance indices were not very useful for predicting heterosis when parental lines belong to different genetic groups: the identification of polymorphisms of genes involved in the traits analysed is required. Second, enzyme markers were used to follow introgression of exotic into adapted material, in an experiment on cold tolerance. The classification observed revealed neither genetic drift nor selection. Finally, using a saturated genetic map constructed from an F2 progeny, we located QTLs (quantitative trait loci) for agromorphological traits and QTLs for amounts of proteins as revealed by 2-dimensional electrophoresis and quantified with a computerassisted system. The comparison of genetic parameters (dominance, epistasis) between these 2 contrasted phenotypic levels suggested that interactions are much more common at the gene product level than at the macroscopic level.
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  • PRODINRA : 160579

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Dominique de Vienne, J.M. Josse, Aurélie Maurice, Mathilde Causse, Adrien Leonardi, et al.. Marquage et expression du genome chez le mais. Colloque BRG/INRA. Ressources génétiques animales et végétales. Méthodologie d'étude et de gestion, Sep 1993, Montpellier, France. ⟨hal-02852369⟩

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