The Escherichia coli bio¢lm-promoting protein Antigen 43 does not contribute to intestinal colonization - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement Access content directly
Journal Articles FEMS Microbiology Letters Year : 2008

The Escherichia coli bio¢lm-promoting protein Antigen 43 does not contribute to intestinal colonization

Abstract

Escherichia coli is a versatile organism capable of causing a variety of intestinal and extraintestinal diseases, as well as existing as part of the commensal flora. A variety of factors permit specific attachment to host receptors including fimbrial adhesins and outer membrane proteins such as autotransporters. One of the better characterized autotransporters is Antigen 43 (Ag43), the major phase-variable surface protein of E. coli. Ag43 is associated with bacterial cell-cell aggregation and biofilm formation. Nevertheless, the precise biological significance and contribution to intestinal colonization remain to be elucidated. Here we investigated the contribution of Ag43 to E. coli adherence to intestinal epithelial cells and colonization of the mouse intestine. These investigations revealed that Ag43 increased in vitro adherence of E. coli to epithelial cells by promoting bacterial cell-cell aggregation but that Ag43 did not promote specific interactions with the mammalian cells. Furthermore, Ag43 did not contribute significantly to coloniza-tion of the mouse intestine and expression of Ag43 was lost a few days after colonization of the mouse was established. Unexpectedly, considering its similarity to other adhesins, our findings suggest that Ag43 does not act as a direct colonization factor by binding to mammalian cells.
Fichier principal
Vignette du fichier
De Luna_FEMSMicLett08.pdf (761.92 Ko) Télécharger le fichier
Origin : Files produced by the author(s)
Loading...

Dates and versions

hal-02910752 , version 1 (03-08-2020)

Identifiers

Cite

Maria das Graã§as de Luna, Anthony Scott-Tucker, Mickaël Desvaux, Paul Ferguson, Nicholas P Morin, et al.. The Escherichia coli bio¢lm-promoting protein Antigen 43 does not contribute to intestinal colonization. FEMS Microbiology Letters, 2008, 284 (2), pp.237-246. ⟨10.1111/j.1574-6968.2008.01207.x⟩. ⟨hal-02910752⟩

Collections

INRA INRAE
8 View
57 Download

Altmetric

Share

Gmail Facebook X LinkedIn More