Skip to Main content Skip to Navigation
Journal articles

The Escherichia coli bio¢lm-promoting protein Antigen 43 does not contribute to intestinal colonization

Abstract : Escherichia coli is a versatile organism capable of causing a variety of intestinal and extraintestinal diseases, as well as existing as part of the commensal flora. A variety of factors permit specific attachment to host receptors including fimbrial adhesins and outer membrane proteins such as autotransporters. One of the better characterized autotransporters is Antigen 43 (Ag43), the major phase-variable surface protein of E. coli. Ag43 is associated with bacterial cell-cell aggregation and biofilm formation. Nevertheless, the precise biological significance and contribution to intestinal colonization remain to be elucidated. Here we investigated the contribution of Ag43 to E. coli adherence to intestinal epithelial cells and colonization of the mouse intestine. These investigations revealed that Ag43 increased in vitro adherence of E. coli to epithelial cells by promoting bacterial cell-cell aggregation but that Ag43 did not promote specific interactions with the mammalian cells. Furthermore, Ag43 did not contribute significantly to coloniza-tion of the mouse intestine and expression of Ag43 was lost a few days after colonization of the mouse was established. Unexpectedly, considering its similarity to other adhesins, our findings suggest that Ag43 does not act as a direct colonization factor by binding to mammalian cells.
Document type :
Journal articles
Complete list of metadata

Cited literature [50 references]  Display  Hide  Download

https://hal.inrae.fr/hal-02910752
Contributor : Stéphanie Dutilloy <>
Submitted on : Monday, August 3, 2020 - 8:15:47 AM
Last modification on : Tuesday, June 22, 2021 - 12:02:02 PM

File

De Luna_FEMSMicLett08.pdf
Files produced by the author(s)

Identifiers

Collections

Citation

Maria das Graças de Luna, Anthony Scott-Tucker, Mickael Desvaux, Paul Ferguson, Nicholas Morin, et al.. The Escherichia coli bio¢lm-promoting protein Antigen 43 does not contribute to intestinal colonization. FEMS Microbiology Letters, Wiley-Blackwell, 2008, 284 (2), pp.237-246. ⟨10.1111/j.1574-6968.2008.01207.x⟩. ⟨hal-02910752⟩

Share

Metrics

Record views

32

Files downloads

57