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Article Dans Une Revue npj Biofilms and Microbiomes Année : 2020

Combining proteogenomics and metaproteomics for deep taxonomic and functional characterization of microbiomes from a non-sequenced host

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Metaproteomics of gut microbiomes from animal hosts lacking a reference genome is challenging. Here we describe a strategy combining high-resolution metaproteomics and host RNA sequencing (RNA-seq) with generalist database searching to survey the digestive tract of Gammarus fossarum, a small crustacean used as a sentinel species in ecotoxicology. This approach provides a deep insight into the full range of biomasses and metabolic activities of the holobiont components, and differentiates between the intestine and hepatopancreatic caecum.
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hal-02922047 , version 1 (04-01-2024)

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Duarte Gouveia, Olivier Pible, Karen Culotta, Virginie Jouffret, Olivier Geffard, et al.. Combining proteogenomics and metaproteomics for deep taxonomic and functional characterization of microbiomes from a non-sequenced host. npj Biofilms and Microbiomes, 2020, 6 (1), pp.7. ⟨10.1038/s41522-020-0133-2⟩. ⟨hal-02922047⟩
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