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Combining proteogenomics and metaproteomics for deep taxonomic and functional characterization of microbiomes from a non-sequenced host

Abstract : Metaproteomics of gut microbiomes from animal hosts lacking a reference genome is challenging. Here we describe a strategy combining high-resolution metaproteomics and host RNA sequencing (RNA-seq) with generalist database searching to survey the digestive tract of Gammarus fossarum, a small crustacean used as a sentinel species in ecotoxicology. This approach provides a deep insight into the full range of biomasses and metabolic activities of the holobiont components, and differentiates between the intestine and hepatopancreatic caecum.
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https://hal.inrae.fr/hal-02922047
Contributor : Davide Degli Esposti <>
Submitted on : Tuesday, August 25, 2020 - 5:21:44 PM
Last modification on : Friday, May 28, 2021 - 1:00:50 PM

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Distributed under a Creative Commons Attribution 4.0 International License

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Duarte Gouveia, Olivier Pible, Karen Culotta, Virginie Jouffret, Geffard Olivier, et al.. Combining proteogenomics and metaproteomics for deep taxonomic and functional characterization of microbiomes from a non-sequenced host. npj Biofilms and Microbiomes, [London?]: Springer Nature published in partnership with Nanyang Technological University, 2020, 6 (1), pp.6. ⟨10.1038/s41522-020-0133-2⟩. ⟨hal-02922047⟩

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