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The Polyamine Spermidine Modulates the Production of the Bacterial Genotoxin Colibactin

Abstract : Colibactin is a polyketide/nonribosomal peptide produced by Escherichia coli strains that harbor the pks island. This toxin induces DNA double-strand breaks and DNA interstrand cross-links in infected eukaryotic cells. Colibactin-producing strains are found associated with colorectal cancer biopsy specimens and promote intestinal tumor progression in various murine models. Polyamines are small polycationic molecules produced by both microorganisms and eukaryotic cells. Their levels are increased in malignancies, where they contribute to disease progression and metastasis. In this study, we demonstrated that the endogenous spermidine synthase SpeE is required for full genotoxic activity of colibactin-producing E. coli. Supplying spermidine in a ΔspeE pks+ E. coli strain restored genotoxic activity. Spermidine is involved in the autotoxicity linked to colibactin and is required for direct damaging activity on DNA. The production of the colibactin prodrug motif is impaired in ΔspeE mutants. Therefore, we demonstrated that spermidine has a direct impact on colibactin synthesis.
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Contributor : Nougayrede Jean-Philippe <>
Submitted on : Friday, September 11, 2020 - 4:19:27 PM
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Camille Chagneau, Christophe Garcie, Nadège Bossuet-Greif, Sophie Tronnet, Alexander Brachmann, et al.. The Polyamine Spermidine Modulates the Production of the Bacterial Genotoxin Colibactin. MSphere, American Society for Microbiology., 2019, 4 (5), pp.00414-19. ⟨10.1128/mSphere.00414-19⟩. ⟨hal-02935548⟩



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