The segregation of the L prolific mutation in the FecL major gene was described in the meat strain of Lacaune sheep. The L mutation was shown to not only affect the prolificacy, but also the fertility after insemination and the growth and mortality of lambs born from L carrier ewes. In order to increase our knowledge about the potential pleiotropic effect of the L prolific mutation, we have observed 20 ++ and 23 L+ Lacaune ewe lambs from birth to adulthood. We have recorded their growth, average daily gain (ADG), endocrine hormone profiles, onset of puberty and fertility after first natural mating. The L mutation did not affect the birth weight or the ADG among the analyzed period. In contrast, these two traits were significantly decreased by the twin or triplet mode of birth compared to single. Interestingly, by checking circulating progesterone level (>0.5ng/ml), L+ ewe lambs have shown precocious ovulation events one month earlier on average than ++ (5 vs. 6, P<0.001). This apparent precocity was not correlated to a precocious set up of a regular cyclicity, but pregnancy rate at first mating of L+ tended to be higher than ++ (95.6% vs. 75.0%, P=0.08). Moreover, we have established the AMH endocrine profile of these lambs as a known marker of the ovarian follicular reserve and possibly fertility. Circulating AMH rises from birth to 2 months of age, decreases from 2 to 3 months and remains stable thereafter. We have found no correlation between AMH profiles and the other recorded parameters. However, L+ ewes have 3 times less AMH than ++, particularly at the time of the concentration rise (P<0.001, 28-70 days), without negative impact on fertility. In conclusion, the L mutation impacts the ovarian function more widely than simply regulating the ovulation rate.