Skip to Main content Skip to Navigation
Journal articles

pH effect on strain-specific transcriptomes of the take-all fungus

Abstract : The soilborne fungusGaeumannomyces tritici(G.tritici) causes the take-all disease on wheat roots. Ambient pH has been shown to be critical in different steps ofG.triticilife cycle such as survival in bulk soil, saprophytic growth, and pathogenicity on plants. There are however intra-specific variations and we previously found two types ofG.triticistrains that grow preferentially either at acidic pH or at neutral/alkaline pH; gene expression involved in pH-signal transduction pathway and pathogenesis was differentially regulated in two strains representative of these types. To go deeper in the description of the genetic pathways and the understanding of this adaptative mechanism, transcriptome sequencing was achieved on two strains (PG6 and PG38) which displayed opposite growth profiles in two pH conditions (acidic and neutral). PG6, growing better at acidic pH, overexpressed in this condition genes related to cell proliferation. In contrast, PG38, which grew better at neutral pH, overexpressed in this condition genes involved in fatty acids and amino acid metabolisms, and genes potentially related to pathogenesis. This strain also expressed stress resistance mechanisms at both pH, to assert a convenient growth under various ambient pH conditions. These differences in metabolic pathway expression between strains at different pH might buffer the effect of field or soil variation in wheat fields, and explain the success of the pathogen.
Document type :
Journal articles
Complete list of metadata

https://hal.inrae.fr/hal-02979591
Contributor : Christine Guillard <>
Submitted on : Tuesday, October 27, 2020 - 11:13:03 AM
Last modification on : Sunday, April 11, 2021 - 7:16:02 PM

Licence


Distributed under a Creative Commons Attribution - NonCommercial - ShareAlike 4.0 International License

Links full text

Identifiers

Citation

Kévin Gazengel, Lionel Lebreton, Nicolas Lapalu, Joëlle Amselem, Anne-Yvonne Guillerm-Erckelboudt, et al.. pH effect on strain-specific transcriptomes of the take-all fungus. PLoS ONE, Public Library of Science, 2020, 15 (7), pp.e0236429. ⟨10.1371/journal.pone.0236429⟩. ⟨hal-02979591⟩

Share

Metrics

Record views

34