Design of a degenerate primer pair to target a bacterial functional community: The hppd bacterial gene coding for the enzyme targeted by herbicides, a study case - Archive ouverte HAL Access content directly
Journal Articles Journal of Microbiological Methods Year : 2020

Design of a degenerate primer pair to target a bacterial functional community: The hppd bacterial gene coding for the enzyme targeted by herbicides, a study case

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Abstract

The present work aimed to design a degenerate primer pair to target a large part of the hppd soil bacterial community, possibly affected by herbicides. We validated these primers by qPCR and high-throughput sequencing analysis of soil samples.

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Environmental Sciences
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Submitted on : Thursday, July 21, 2022-10:26:04 AM

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hal-03130404 , version 1 (21-07-2022)

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Attribution - NonCommercial - CC BY 4.0

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Elsevier-hosted Accepted Manuscript

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C. Thiour-Mauprivez, Marion Devers-Lamrani, A. Mounier, Jérémie Beguet, A. Spor, et al.. Design of a degenerate primer pair to target a bacterial functional community: The hppd bacterial gene coding for the enzyme targeted by herbicides, a study case. Journal of Microbiological Methods, 2020, 170, pp.105839. ⟨10.1016/j.mimet.2020.105839⟩. ⟨hal-03130404⟩

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UNIV-BOURGOGNE CNRS LBBM EDF SORBONNE-UNIVERSITE SU-SCIENCES UMS-2348 INRAE AGROECOLOGIE
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