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Polyclonal Antibody Effects on the Human Cardiac 5-HT4(e) Receptors Depend Upon the Expression System

Abstract : The initial objective of this work was to examine the effects of an antibody (Anti-G21V) directed against the second extracellular loop of human heart 5-HT4 receptors expressed in Chinese hamster ovary (CHO) cells. The antibody anti-G21V had no effect upon either basal cAMP- or 5-HT-evoked increases in cAMP in CHO cells, whereas it had shown an agonist-like effect in COS-7 cells. Analysis of agonist fractions of h5-HT4(e) receptors in CHO and COS-7 cells revealed that equilibrium constant could underlie the different responses of the receptor toward the anti-G21V antibody. Therefore, different expression systems could give rise to functional differences in 5-HT4 receptor behavior.
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https://hal.inrae.fr/hal-03170864
Contributor : Eric Marchoux <>
Submitted on : Tuesday, March 16, 2021 - 2:58:08 PM
Last modification on : Friday, April 9, 2021 - 12:54:06 PM

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Emmanuella Scala, Stéphanie Rose, Olivier Hérault, Jorge Argibay, Pierre Cosnay, et al.. Polyclonal Antibody Effects on the Human Cardiac 5-HT4(e) Receptors Depend Upon the Expression System. Receptors and Channels, Informa UK (Taylor & Francis), 2004, 10 (3-4), pp.125-129. ⟨10.1080/10606820490514950⟩. ⟨hal-03170864⟩

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