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Ribosomal proteins of Tetrahymena thermophila. Correlation of one-and two-dimensional electrophoretic migration patterns and characterization of additional small and large subunit proteins

Abstract : Further analysis of the protein complement of the cytoplasmic ribosome of the protozoon Tetrahymena thermophila has led to the identification and characterization of seven additional proteins, three in the small and four in the large subunit of this ribosome. Several of these proteins are poorly soluble or insoluble in the absence of high concentrations of urea and are not seen in the electrophoretic distribution patterns of ribosomal proteins in two-dimensional polyacrylamide gels unless 6 M urea is added to electrode buffers in contact with protein samples (first dimension) and first-dimension gels (second dimension). The migration patterns of the 40S and 60S subunits of the T. thermophila ribosome in one-dimensional polyacrylamide SDS gels and in two-dimensional gels prepared by means of the basic-acidic system of Kaltschmidt and Wittmann**, and the basic-SDS system of Zinker and Warner*** have been correlated.
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https://hal.inrae.fr/hal-03187316
Contributor : Barbara Petridou <>
Submitted on : Wednesday, March 31, 2021 - 10:35:07 PM
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B PETRIDOU 1989 Biochimie 71, ...
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Françoise Hayes, Barbara Petridou, Marie-France Guerin, Franqoise Hayes. Ribosomal proteins of Tetrahymena thermophila. Correlation of one-and two-dimensional electrophoretic migration patterns and characterization of additional small and large subunit proteins. Biochimie, Elsevier, 1989, 71 (5), pp.655-665. ⟨10.1016/0300-9084(89)90160-0⟩. ⟨hal-03187316⟩

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