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ABCG transporters export cutin precursors for the formation of the plant cuticle

Abstract : The plant cuticle is deposited on the surface of primary plant organs, such as leaves, fruits, and floral organs,forming a diffusion barrier and protecting the plant against various abiotic and biotic stresses. Cutin, thestructural polyester of the plant cuticle, is synthesized in the apoplast. Plasma-membrane-localized ATP-binding cassette (ABC) transporters of the G family have been hypothesized to export cutin precursors.Here, we characterizeSlABCG42of tomato representing an ortholog ofAtABCG32inArabidopsis.SlABCG42expression inArabidopsiscomplements the cuticular deficiencies of theArabidopsis pec1/abcg32mutant.RNAi-dependent downregulation of both tomato genes encoding proteins highly homologous to AtABCG32(SlABCG36 and SlABCG42) leads to reduced cutin deposition and formation of a thinner cuticle in tomatofruits. By using a tobacco (Nicotiana benthamiana) protoplast system, we show that AtABCG32 andSlABCG42 have an export activity for 10,16-dihydroxy hexadecanoyl-2-glycerol, a cutin precursorin vivo.Interestingly, also freeu-hydroxy hexadecanoic acid as well as hexadecanedioic acid were exported,furthering the research on the identification of cutin precursorsin vivoand the respective mechanisms of their integration into the cutin polymer.
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https://hal.inrae.fr/hal-03241334
Contributor : Olivier Dupre <>
Submitted on : Friday, May 28, 2021 - 3:35:00 PM
Last modification on : Tuesday, September 7, 2021 - 3:40:01 PM

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Carolina Elejalde-Palmett, Ignacio Martinez San Segundo, Imène Garroum, Laurence Charrier, Damien de Bellis, et al.. ABCG transporters export cutin precursors for the formation of the plant cuticle. Current Biology - CB, Elsevier, 2021, 31 (10), pp.2111-2123.e9. ⟨10.1016/j.cub.2021.02.056⟩. ⟨hal-03241334⟩

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