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A two-step morphology-PCR strategy for the identification of nematode larvae recovered from muscles after artificial digestion at meat inspection

Abstract : To ensure that meat from livestock and game is safe for human consumption, European legislation lays down rules for mandatory testing. Helminth larvae are a category of zoonotic foodborne pathogens that can contaminate meat. Among helminths, the only zoonotic nematode regulated in Europe regarding meat inspection isTrichinellaspp.. It is precisely during Trichinella testing that other potentially zoonotic larvae can be found. Due to current lack of tools, their identification is often very complicated. Nematode larvae other thanTrichinella, recovered from artificial digestions of pig and wild boar muscles from France and Germany, were subjected to a newly developed two-step identification scheme, which includes both morphological examination and molecular assays. The first step is a general orientation towards a broad taxonomic group; the second step consists of targeted identification based on the results of first step. Different parasites were identified, some of which were not zoonotic such asMetastrongylusspp. andAngiostrongylus vasorum, but others are known to be zoonotic such asToxocara cati,Ascaris suum, andUncinaria stenocephala. The strategy is efficient for the identification of nematode larvae recovered from muscles but could also be applied for larvae from other sources.
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https://hal.inrae.fr/hal-03316740
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Submitted on : Friday, August 6, 2021 - 3:22:10 PM
Last modification on : Thursday, September 23, 2021 - 11:02:03 AM

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Grégory Karadjian, Carolyn Kaestner, Lisa Laboutière, Emilie Adicéam, Tom Wagner, et al.. A two-step morphology-PCR strategy for the identification of nematode larvae recovered from muscles after artificial digestion at meat inspection. Parasitology Research, Springer Verlag (Germany), 2020, 119 (12), pp.4113-4122. ⟨10.1007/s00436-020-06899-7⟩. ⟨hal-03316740⟩

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