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Development and validation of a lateral flow immunoassay for rapid detection of VanA-producing enterococci

Abstract : Background: VRE are nosocomial pathogens with an increasing incidence in recent decades. Rapid detection is crucial to reduce their spread and prevent infections and outbreaks. Objectives: To evaluate a lateral flow immunoassay (LFIA) (called NG-Test VanA) for the rapid and reliable detection of VanA-producing VRE (VanA-VRE) from colonies and broth. Methods NG-Test VanA was validated on 135 well-characterized enterococcal isolates grown on Mueller–Hinton (MH) agar (including 40 VanA-VRE). Different agar plates and culture broths widely used in routine laboratories for culture of enterococci were tested. Results: All 40 VanA-VRE clinical isolates were correctly detected in less than 15 min irrespective of the species expressing the VanA ligase and the medium used for bacterial growth. No cross-reaction was observed with any other clinically relevant ligases (VanB, C1, C2, D, E, G, L, M and N). Overall, the sensitivity and specificity of the assay were 100% for VanA-VRE grown on MH agar plates. NG-Test VanA accurately detects VanA-VRE irrespective of the culture medium (agar and broth). Band intensity was increased when using bacteria grown on vancomycin-containing culture media or on MH close to the vancomycin disc as a consequence of VanA induction. The limit of detection of the assay was 6.3 × 106 cfu per test with bacteria grown on MH plates and 4.9 × 105 cfu per test with bacteria grown on ChromID® VRE plates. Conclusions: NG-Test VanA is efficient, rapid and easy to implement in the routine workflow of a clinical microbiology laboratory for the confirmation of VanA-VRE.
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https://hal.inrae.fr/hal-03321752
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Submitted on : Wednesday, August 18, 2021 - 9:52:28 AM
Last modification on : Friday, August 20, 2021 - 9:15:07 AM

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Saoussen Oueslati, Hervé Volland, Vincent Cattoir, Sandrine Bernabeu, Delphine Girlich, et al.. Development and validation of a lateral flow immunoassay for rapid detection of VanA-producing enterococci. Journal of Antimicrobial Chemotherapy, Oxford University Press (OUP), 2021, 76 (1), pp.146-151. ⟨10.1093/jac/dkaa413⟩. ⟨hal-03321752⟩

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