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Poster communications

Impact of polyphenols on the microbiota-gut-brain axis -Study of 5-O-caffeoylquinic acid and its gut bacterial-derived metabolites

Abstract : Aim: Polyphenols, dietary compounds, have drawn increasing attention due to their ability to lower oxidative stress involved in many pathologies and due to their positive effects in modulation of redox cellular signalling pathways [1]. The objectives of our studies are to identify the compounds derived from polyphenols after cellular metabolization (neuronal cells and gut bacteria) and to highlight how these molecules can act beneficially on microbiota and prevent neurodegenerescence diseases [2]. A particular attention is paid on oxidized phenolic metabolites and their biological effects. Methods: Currently, we focus our work in the impact of 5-O-caffeoylquinic acid (5-CQA), a dietary phenolic compound with neuroprotective properties [3], on the growth and the metabolism of three intestinal bacterial species (Lactobacillus reuteri, Bacteroides fragilis and Bifidobacterium longum). In parallel, we determine the bioconversion of 5-CQA by these three bacteria in mono and co-cultures. We particularly use LC-ESI-MS 2 and a molecular networking approach (GNPS platform) [4] to identify the biotransformed metabolites (e.g. oxidized derivatives). In addition, electrochemical technics are helpful to study the redox mechanism of 5-CQA and to generate oxidized samples. Results: L. reuteri has shown capacity to biotransform 5-CQA notably into caffeic acid and its oxidized derivative quinone, and into 3-hydroxybenzoic acid, contrary to B. fragilis and B. longum which didn't show any ability. First experiments in co-cultures were also carried out. Conclusion and perspectives: We have demonstrated an oxidative pathway in the bioconversion of 5-CQA by L. reuteri. Further experiments will be carried out to show the role of native, oxidized and biotransformed dietary polyphenols in the gut-brain axis.
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Poster communications
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https://hal.inrae.fr/hal-03325742
Contributor : Sylvain Guyot <>
Submitted on : Wednesday, August 25, 2021 - 11:34:00 AM
Last modification on : Monday, August 30, 2021 - 10:27:16 AM

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Xiaoxi Yu, Hélène Sotin, Nathalie Marnet, Sylvain Guyot. Impact of polyphenols on the microbiota-gut-brain axis -Study of 5-O-caffeoylquinic acid and its gut bacterial-derived metabolites. EDUC (European Digital UniverCity) - Lifelong Health and Wellbeing scientific seminar, Mar 2021, Pécs, Hungary. ⟨hal-03325742⟩

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